2019年8月，我校莊松林院士團(tuán)隊(duì)超精密光學(xué)制造課題組張大偉教授、戴博副教授、鄭璐璐老師、萬新軍老師、焦子傲同學(xué)的最新成果論文《Colour compound lenses for a portable fluorescence microscope》在國(guó)際光學(xué)頂尖期刊《Light: Science & Applications》（影響因子14.098）上發(fā)表。戴博、焦子傲和鄭璐璐為第一作者，張大偉和Tony Jun Huang為通訊作者。
該成果與美國(guó)杜克大學(xué)、美國(guó)戴頓大學(xué)、復(fù)旦大學(xué)、復(fù)旦大學(xué)附屬腫瘤醫(yī)院合作，主要研制了一種彩色液滴透鏡器件，并實(shí)現(xiàn)了基于智能手機(jī)的便攜式熒光顯微成像應(yīng)用。
熒光顯微成像技術(shù)是實(shí)現(xiàn)生物樣品觀測(cè)與生化指標(biāo)檢測(cè)的重要方式之一，傳統(tǒng)的熒光顯微檢測(cè)只能在實(shí)驗(yàn)室利用昂貴的熒光顯微鏡實(shí)現(xiàn)，為了解決設(shè)備昂貴、攜帶不便等問題，研究人員受到“葡萄酒濾光現(xiàn)象”的啟發(fā)，利用蒽醌染料對(duì)高分子聚合物和高折射率硅油進(jìn)行染色，通過光流控技術(shù)，創(chuàng)新制作了多款彩色復(fù)合液滴透鏡，該透鏡可同時(shí)實(shí)現(xiàn)圖像放大及光學(xué)濾波的功能。并且，通過將透鏡安裝于手機(jī)上，實(shí)現(xiàn)了細(xì)胞觀察、細(xì)胞計(jì)數(shù)、腫瘤組織切片熒光檢測(cè)、質(zhì)粒轉(zhuǎn)染表達(dá)檢測(cè)、細(xì)胞凋亡超氧化物生成檢測(cè)等應(yīng)用，得到了同行專家的高度認(rèn)可。該研究成果可廣泛用于生物醫(yī)學(xué)即時(shí)檢測(cè)的應(yīng)用環(huán)境，為醫(yī)療資源匱乏地區(qū)以及戰(zhàn)場(chǎng)醫(yī)學(xué)提供有效技術(shù)保障。
該研究項(xiàng)目得到了國(guó)家重點(diǎn)研發(fā)計(jì)劃、國(guó)家自然科學(xué)基金、上海市科學(xué)技術(shù)委員會(huì)項(xiàng)目的資助。
圖1彩色液滴透鏡及便攜式熒光顯微鏡
圖2小鼠黑色素瘤細(xì)胞細(xì)胞及腫瘤組織切片觀察
論文鏈接：https://www.nature.com/articles/s41377-019-0187-1
B. Dai, Z. Jiao, L. Zheng, H. Bachman, Y. Fu, X. Wan, Y. Zhang, Y. Huang, X. Han, C. Zhao, T. J. Huang*, S. Zhuang, D. Zhang*, “Colour compound lenses for a portable fluorescence microscope,” Light: Science & Applications, vol. 8, article no. 75, Aug. 2019.
Editorial Summary
Fluorescence microscopy: Smartphone solutions for seeing cells
A method of turning a smartphone into a fluorescence microscope, developed by researchers in the US and China, enables complex biomedical analyses to be performed rapidly and inexpensively. Conventional fluorescence microscopes are vital for detecting specific cell types and proteins, but are bulky and inconvenient for point-of-care diagnoses. Tony Jun Huang at Duke University, Dawei Zhang at USST and co-workers used liquid polymers to create miniature lenses comprising two droplets, one inside the other, dyed with colored solvents. The lenses, which are compatible with several different smartphone cameras, allowed the researchers not only to observe and count cells, but also to monitor the expression of fluorescently-tagged genes, and to distinguish between normal tissue and tumors. This ingenious use of easily-accessible and affordable smartphone technology will lead to better on-site personalized medicine, especially for developing countries.
供稿：光電學(xué)院
